Sign
A sign could be anything to communicate and it come in any sort of form. It can be Spoken, Shown or even smelled. A toilet sign is a basic sign that communicates to us where we can get... a toilet. It was invented by Samuel M. Genensky.
Symbolic signs
These are the signs that are purely about relation between the signifier and signified. They can vary between different cultures. Here's a great advert by HSBC that simply explains it.
Denotation
Denotation is what does literally sign mean. For example, an apple is a fruit.
Connotation
Connotation gives some sort of meaning to a word. It could be giving an emotion to a sign and connotation usually differs culturally. For example an apple in United Kingdom could mean 'healthy' while in Africa it could be look as survival from hunger.
Sources:
University of Vermont, [online], available at: http://www.uvm.edu/~tstreete/semiotics_and_ads/terminology.html [accessed: 10/05/13]
Image Links:
http://img2.etsystatic.com/004/0/6000195/il_fullxfull.365434374_tscn.jpg
https://blogger.googleusercontent.com/img/b/R29vZ2xl/AVvXsEjFbY-PEaS4OUWP6bry-sxDOSH7vBdFnfjpUgCb578xeK-0SIzicqTECOAeE0dLPvnWxiveEcndrTJoVcmwol8qPShh-AS4Hhp-qDrGuVyLydO1C91a_pBKFjBydSjcgLieb9mdXaE-2tsc/s1600/apple2.jpg
http://www.crcl.net/images/page/89_boy_taffle_apple.jpg
Video Link:
http://www.youtube.com/watch?v=rZ-qFNrrt0E

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